MICROBIOLOGICAL AND BIOCHEMICAL EVALUATION OF BACTERIA PRESENT IN PERIAPICAL LESIONS IN PATIENTS WITHOUT SYSTEMIC DISEASES

Abstract

The periapical lesions are immunoinflammatory reactions, which comprise an integrated network with bacterial virulence factors and therefore an intrinsic infectious process is installed, resulting in a microenvironment providing a periapical tissue destruction in a devitalized tooth. The objective of this study was to evaluate the bacterial population present in periapical lesions in patients without systemic diseases. The patients (n = 5) signed an Informed Consent (IC) and the methodology used for bacterial identification, followed by default, the morphological characteristics of the colonies in the culture medium (blood agar) and biochemical tests specific, and the clinical samples were obtained through tooth extraction and mechanical curettage of the lesion. Among the five samples tested had as a result, 100% of facultative anaerobic bacteria and Gram-positive cocci, the following bacterial species being found in decreasing order of prevalence: Streptococcus viridans (71%), Staphylococcus aureus (17%), Staphylococcus negative coagulase (11%) and Enterococcus faecalis (1%). Through this research we can conclude that there is a higher prevalence of S. viridans in periapical lesions, in addition, bacterial identification by means of the morphological characteristics of the colonies in the culture medium and the specific biochemical tests are effective.