EVALUATION OF THE DNA AND SECONDARY METABOLITE PRO-DUCTION BY THE FUNGUS TRICHODERMA REESEI DURING ITS GROWTH PHASES

Abstract

The genus Trichoderma is of great interest in agriculture and medicine for producing bioactive compounds applied in the industry. The objective of this paper was to evaluate the production of secondary metabolites in the different growth stages of Trichoderma reesei, its antimicrobial activity and evaluate in house DNA extraction of T. reesei, using the Salting out protocol. A culture with 10⁵ spores was cultivated in Sabouraud broth, and it was monitored for 7 days. For the process of metabolites production, the secondary metabolite was evaluated in spectrophotometer between the wavelength of 200nm to 600nm. The antimicrobial function was assessed by agar diffusion test. The synthesis of the secondary metabolite production was assessed by the production of a yellow exudate. The antibiotic susceptibility presented an inhibition zone in treatments 30°C and 37°C + COâ‚‚for Proteus spp. and treatment 37°C for S. saprophyticcus, S. epidermidis and Salmonella spp. The RT treatment presented no inhibition zone for the tested bacteria. Results exhibit obtainment of good quality genomic DNA in treatments T1, T2, T3 and T4, presenting satisfactory integrity, concentration and high purity. T5, T6 and T7 treatments, that used for spores for the DNA extraction, presented no satisfactory results.